Over the past third of a century, the Vancouver Aquarium, under the guidance of Dr. Jeff Marliave, has earned accolades from the zoological and research communities for its first-time rearing of cold water marine species such as wolf-eels, pandalid shrimp and grunt sculpins. The Aquarium's breeding program has supplied such popular cold water ornamentals as wolf- eels, Pacific spiny lumpsuckers, and grunt and sailfin sculpins to aquarium facilities worldwide.
In recent years, culturing research at the Aquarium has centered on the dietary requirements of larval and juvenile fish. Today, a total of 60 fish and 16 crustacean species have been reared in the Aquarium laboratory.
| Species | Common Name | Year | Details |
| Agonopsis vulsa | Northern spearnose poacher | 1996 | reared 1st time |
| Agonus acipenserinus | Sturgeon poacher | 1996 | reared 1st time |
| Anarrhichthys ocellatus | Wolf-eel | 2001 | reared on pellets |
| Anoplarchus pupurescens | Cockscomb prickleback | 1997 | reared re/ hybridization |
| Anoplarchus purpurescens X insignis | Hybrid blenny-eels | 2006 | reared (died before juvenile) |
| Apodichthys flavidus | Penpoint gunnels | 2000 | reared |
| Artedius fenestralis | Padded sculpin | 1998 | reared |
| Asemichthys taylori | Spinynose sculpin | 1996 | reared 2nd time |
| Aulorhynchus flavidus | Tubesnout | 2006 | F8 |
| Chirolophis decoratus | Decorated warbonnet | 2004 | reared |
| Chirolophis nugator | Mosshead warbonnet | 1997 | reared 1st time |
| Chitonotus pugetensis | Roughback sculpin | 2000 | F2 |
| Clinocottus globiceps | Mosshead sculpin | 2002 | reared 1st time |
| Clupea harengus pallasi | Pacific herring | 1997 | reared re/ food and display |
| Enophrys bison | Buffalo sculpin | 1997 | reared re/ nesting parasitism |
| Eumicrotremus orbis | Pacific spiny lumpsucker | 2003 | reared |
| Gasterosteus aculeatus | Threespine stickleback | 2001 | F2 |
| Heptacarpus kincaidi | Kincaid's shrimp | 2002 | reared |
| Hexagrammos lagocephalus | Rock greenling | 1997 | reared 1st time |
| Hexagrammos stelleri | Whitespotted greenling | 2000 | reared |
| Hippocampus abdominalis | Pot-bellied seahorse | 2005 | reared |
| Hippocampus erectus | Lined seahorses | 2001 | F5 |
| Hippocampus ingens | Giant seahorse | 1998 | F4 |
| Icelinus borealis | Northern sculpin | 1996 | reared 1st time (complete development series) |
| Jordania zonope | Longfin sculpin | 1997 | F2 |
| Lebbeus grandimanus | Candystripe shrimp | 2002 | F4 |
| Lebbeus schrencki | Okhotsk lebbeid shrimp | 2003 | F7 |
| Liparis cyclopus | Ribbon snailfish | 1998 | reared 1st time (complete development series) |
| Lopholithodes mandtii | Puget Sound king crab | 1997 | reared 1st time (to juvenile stage only) |
| Metacrangon munita | Coastal spinyhead shrimp | 1997 | reared 1st time |
| Nautichthys oculofasciatus | Sailfin sculpin | 1997 | reared |
| Odontopyxis trispinosa | Pygmy poacher | 1996 | reared 1st time |
| Oligocottus maculosus | Tidepool sculpin | 2001 | reared |
| Oligocottus snyderi | Fluffy sculpin | 1997 | F2 |
| Ophiodon elongatus | Lingcod | 1996 | reared re/ transplant study |
| Oxylebius pictus | Painted greenling | 2006 | reared |
| Paguras armatus | Black-eyed hermit crab | 1996 | reared 1st time |
| Pandalopsis lucidirimicola | Sparkling shrimp | 1997 | reared 1st time (undescribed species) |
| Pandalus danae | Coonstripe shrimp | 1998 | reared (previously domesticated) |
| Pandalus eous | Spiny pink shrimp | 1998 | reared |
| Pandalus hypsinotus | Dock shrimp | 1998 | reared |
| Pandalus platyceros | Spot prawn | 1998 | reared |
| Pandalus stenolepis | Roughpatch shrimp | 1997 | reared 1st time |
| Paracrangon echinata | Horned shrimp | 1997 | reared 1st time |
| Rhamphocottus richardsoni | Grunt sculpin | 2006 | reared |
| Scorpaenichthys marmoratus | Cabezon | 1998 | reared 2nd time |
| Sebastes caurinus | Copper rockfish | 2006 | reared 1st time |
| Syngnathus griseolineatus | Bay pipefish | 1998 | reared |
| Triglops macellus | Roughspine sculpin | 1996 | reared 1st time |
| Xiphister mucosus | Rock prickleback | 2006 | reared |